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| Plano de Ação: | ..... |
| Descrição: | MENDES, L. W.; MENDES, R.; RAAIJMAKERS, J. M.; TSAI, S. M. Breeding for soil-borne pathogen resistance impacts active rhizosphere microbiome of common bean. The ISME Journal, v. 12, p. 3038–3042, (2018). [Cadastro no Ainfo condicionado ao acesso à versão definitiva/paginada] |
| Parceria/partes: | Netherlands Institute of Ecology - NIOO-KNAW, CENA/USP - Centro de Energia Nuclear na Agricultura, |
| Conteúdo: | Over the past century, plant breeding programs have substantially improved plant growth and health, but have not yet considered the potential effects on the plant microbiome. Here, we conducted metatranscriptome analysis to determine if and how breeding for resistance of common bean against the root pathogen Fusarium oxysporum (Fox) affected gene expression in the rhizobacterial community. Our data revealed that the microbiome of the Fox-resistant cultivar presented a significantly higher expression of genes associated with nutrient metabolism, motility, chemotaxis, and the biosynthesis of the antifungal compounds phenazine and colicin V. Network analysis further revealed a more complex community for Fox-resistant cultivar and indicated Paenibacillus as a keystone genus in the rhizosphere microbiome. We suggest that resistance breeding in common bean has unintentionally co-selected for plant traits that strengthen the rhizosphere microbiome network structure and enrich for specific beneficial bacterial genera that express antifungal traits involved in plant protection against infections by root pathogens. |
| Observaçao: | ISSN: 1751-7362 (print), 1751-7370 (online). DOI: https://doi.org/10.1038/s41396-018-0234-6 Nota: Brief Communication. Pendência: código SEG associado. (ID_PAT: 22858) |
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| Plano de Ação: | 03.16.04.008.00.03 |
| Descrição: | MELO, M. P. de; CABRAL, C. S.; REIS, A.; MATOS, K. S.; MARTINS, P. P.; BESERRA JUNIOR, J. E. A.; NECHET, K. de L.; HALFELD-VIEIRA, B. A. Rhizoctonia solani AG 1-IB and AG 4 HG-I causing leaf blight and root rot in plants from the Lamiaceae family in Brazil. Tropical Plant Pathology, v. 43, n. 2, p. 152-159, 2018. |
| Parceria/partes: | Embrapa Hortaliças (CNPH), UFPI - Universidade Federal do Piauí, Teresina/PI, UFRR - Universidade Federal de Roraima, UnB - Universidade de Brasília, |
| Conteúdo: | Abstract: Rhizoctonia solani is a fungal complex represented by 14 anastomosis groups. Each group has distinct characteristics that are reflected in the management and control strategies for diseases caused by this fungus. In three distinct geographical regions, plants of the Lamiaceae family from vegetable and medicinal herb gardens were observed, with Mentha piperina, Mentha spicata and Origanum manjerona presenting symptoms of leaf blight and Ocimum basilicum, Ocimum vulgare and Rosmarinus officinalis presenting root rot symptoms. Mycelia, hymenia, basidiospores and microsclerotia were found on the stems and leaves of Mentha piperina and Mentha spicata. These are typical morphological markers of the genus Rhizoctonia. The ITS region was amplified for nine isolates and subsequently sequenced. In the phylogenetic analysis, the isolates were identified as Rhizoctonia solani belonging to AG 1-IB and AG 4 HG-I anastomosis groups. All isolates inoculated in their respective hosts caused disease symptoms resembling those typically observed in the field. This is the first report of R. solani AG 1-IB in M. spicata, M. piperina and Origanum majorana and of R. solani AG 4 HG-I in O. vulgare, R. officinalis and O. basilicum in Brazil. |
| Observaçao: | DOI: 10.1007/s40858-017-0181-1. (ID_PAT: 21755) |
| Palavras-chave: | Basidiomycetes; Root rot; Medicinal plants; Leaf blight; Anastomosis group. |
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| Plano de Ação: | 03.16.04.008.00.03 |
| Descrição: | BOARI, A. de J.; QUADROS, A. F. F.; NECHET, K. de L. Rhizoctonia solani AG1-IA causing leaf blight in Erythrina indica var. picta in Brazil. Summa Phytopathologica, Botucatu, v. 44, n. 3, p. 293-294, 2018. |
| Parceria/partes: | Embrapa Amazônia Oriental (CPATU), UFRA - Universidade Federal Rural da Amazônia, |
| Conteúdo: | Erythrina indica var. picta (L.) B. & M. (Fabaceae), commonly known as brasileirinho, sunshine tree or coral tree, is a rustic ornamental tree widely used in gardens in Brazil. In March of 2014, leaf blight and intense defoliation in E. indica var. picta were observed in Moju, PA, Brazil (Fig. 1A). The spots were characterized by the presence of mycelia on the leaf surface (Fig. 1A). Microscopic examination of the mycelia revealed association with Rhizoctonia spp. The characteristics observed were a distinct right-angle branching pattern, the constriction of hyphae near the point of origin, and multinucleate individual hyphae compartments (1). The fungus was isolated from infected tissues on potato-dextrose-agar (PDA). The anastomosis group (AG) was determined by pairing the isolate with the following tester strains of multinucleate Rhizoctonia solani group: AG1- IA (CMAA 908) AG1-IB (CMAA 909); AG-2-2 IIIB (CMAA 907); AG3 (CMAA 906); AG4 AHI (CMAA 903); AG4 140 (CMAA 904); AG7-H0 (CMAA 901); AG-BI TS-2-4 (CMAA 905) (2). The isolates were obtained from the Culture Collection of Microorganisms of Environmental and Agricultural Importance (CMAA) of the Brazilian Agricultural Research Corporation, located in Jaguariúna, São Paulo. The isolate of E. indica var. picta anastomosed with AG1-IA. The genomic DNA of the isolate was extracted (3), and the ITS-5.8S region was amplified by using the pair of primers ITS4B/ITS1F. The amplicons were cloned by using pGEM-T Easy Vector System (Promega) kit and the competent Escherichia coli cells OneShot® DH5aTM (Invitrogen, San Diego), which were then used in the PCR reaction. The sequencing reactions were done in Applied Biosystems® 3500 Genetic Analyzer. The pathogenicity test was performed by inoculating healthy leaves with mycelium discs of the isolate. The control leaves were inoculated with PDA discs. After inoculation, the leaves were covered with plastic bags for 24 h and maintained at 28 oC. After five days, foliar necrosis was observed only on inoculated leaves (Fig. 1B). The fungus was reisolated fulfilling Koch´s postulates. The isolate sequence (715 bp) was deposited in the GenBank (access: KP059879) with 99% identity with R. solani accesses from beet (FJ492099) and rice (EF429212) AG1-IA. In the phylogenetic tree of the ITS-5.8S region, the isolate was clustered with R. solani isolates belonging to AG1-IA (Bayesian posterior probability = 1) (Fig. 1C). The isolate was deposited in CMAA (access code CMAA 1586). The results of this study confirmed the fungus R. solani AG1-IA as the etiological agent of E. indica var. picta leaf blight in Brazil. In the Amazon region, the occurrence of R. solani AG1-IA has been reported in important cultures, such as soybean (4), rice (5), and cowpea (6). The current studies of genetic population of R. solani AG1 IA suggest its potential to emerge as a pathogen in new hosts based on the mixed reproductive system of the pathogen, including both sexual reproduction and long-distance dispersal of adapted clones, its genetic diversity and population size (7). These characteristics can support the origin of the emergence of R. solani AG1 IA in Erythrina indica var. picta. |
| Observaçao: | ISSN: 0100-5405 (Print ), 1980-5454 (Online). Acessível em http://dx.doi.org/10.1590/0100-5405/181932 (ID_PAT: 22414) |
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| Valor JCR (2017): | Não se aplica |
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| Plano de Ação: | 16.00.2.50.01.16 |
| Descrição: | VENTURA, A. S.; PÁDUA, S. B. de; ISHIKAWA, M. M.; MARTINS, M. L.; TAKEMOTO, R. M.; JERÔNIMO, G. T. Endoparasites of Gymnotus sp. (Gymnotiformes: Gymnotidae) from commercial baitfish farming in Pantanal basin, Central Brazil. Boletim do Instituto de Pesca, São Paulo, v. 44, n. 3, e332, 2018. 5 p. |
| Parceria/partes: | UEM - Universidade Estadual de Maringá/PR, UFSC - Universidade Federal de Santa Catarina, Aquivet Saúde Aquática, Universidade Nilton Lins, UFMS - Universidade Federal de Mato Grosso do Sul, |
| Conteúdo: | Abstract: This study evaluated the endohelminthic parasites of native baitfish Gymnotus spp. collected from different baitfish farming in the Pantanal basin, State of Mato Grosso do Sul, Central Brazil. From 99 fish collected for parasitological analysis, all of them were found to be parasitized by at least 1 parasite per host. A total of 4504 parasites were collected comprising the following taxa: Digenea, Nematoda, Pentastomida and Acanthocephala. Rondonia rondoni and Goezia sp. as well as Alofia sp. Were registered for the first time in farmed Gymnotus spp. The parasite species diversity in the host is discussed in this study.
Resumo: Este estudo avaliou os parasitos endohelmintos do peixe nativo Gymnotus spp. coletado de diferentes isqueiros comerciais na bacia do Pantanal, Mato Grosso do Sul, Brasil Central. De 99 peixes coletados para análise parasitológica, todos estavam parasitados por pelo menos um parasito por hospedeiro. Um total de 4504 parasitos foram coletados compreendendo os seguintes táxons: Digenea, Nematoda, Pentastomida e Acanthocephala. Rondonia rondoni e Goezia sp. bem como Alofia sp. foram registrados pela primeira vez em Gymnotus spp. A diversidade de espécies de parasitos no hospedeiro é discutida neste estudo. |
| Observaçao: | DOI: 10.20950/1678-2305.2018.322 Publicação associada ao Projeto co-financiado Fundect - edital: 14/2009 – Universal. Término: 31/08/2014, código Embrapa: 03.11.99.054.00.03. (ID_PAT: 22074) |
| Palavras-chave: | Biodiversity; Fish parasites; Gymnotiformes; Neotropics.
Biodiversidade; Parasitos peixes; Gymnotiformes; Neotropical. |
| Valor JCR (2017): | Não se aplica |