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| Plano de Ação: | 16.00.2.50.01.16 |
| Descrição: | NAKASONE, A. K.; CARDOSO, S. V. D.; COUTINHO, I. B. de L.; NECHET, K. de L.; FERREIRA, S. da C.; BOARI, A. de J.; NASCIMENTO, W. M. O. do; CARVALHO, J. E. U. de. Ocorrência de Lasiodiplodia pseudotheobromae em bacurizeiro (Platonia insignis). Summa Phytopathologica, Botucatu, v. 46, n. 1, p. 58-59, 2020. |
| Parceria/partes: | Embrapa Amazônia Oriental (CPATU), UFRA - Universidade Federal Rural da Amazônia, |
| Observaçao: | ISSN: 0100-5405 (impresso), 1980-5454 (online). DOI: http://dx.doi.org/10.1590/0100-5405/193189. Publicação associada à Atividade SEG 12.14.01.023.00.05.001, do Projeto Convertido: Tecnologias para o cultivo sustentável de bacurizeiro, camucamuzeiro, cajazeira e muricizeiro - melhorFRUTA fase III, liderado pela Embrapa Amazônia Oriental (CPATU). (ID_PAT: 24584) |
| Palavras-chave: | Lasiodiplodia pseudotheobromae. |
| Valor JCR (2019): | Não se aplica |
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| Plano de Ação: | 16.00.2.50.01.16 |
| Descrição: | BATISTA, I. C. A.; BOARI, A. de J.; KAUFFMANN, C. M.; NECHET, K. de L. Colletotrichum plurivorum causes anthracnose on okra in Brazil. Journal of Plant Pathology, v. 102, n. 4, article 1331, 2020. |
| Parceria/partes: | Embrapa Amazônia Oriental (CPATU), UFRA - Universidade Federal Rural da Amazônia, UFV - Universidade Federal de Viçosa, |
| Conteúdo: | Abstract: Okra (Abelmoschus esculentus) is a tropical vegetable cultivated widely in the Amazon region. In August 2016, typical anthracnose symptoms were observed on the leaves of okra in Altamira, Pará, Brazil with 60% disease incidence. Symptomatic leaf tissue were surface sterilized, rinsed with sterile water, and plated on potato dextrose agar. Colonies had greyish white aerial mycelium in 7-day-old cultures at 25 ± 2 °C. Conidiophores were smooth-walled, septate. Conidiogenous cells were hyaline, straight, and 19 × 4 μm. Appressoria were single, elliptical, and 8.5 × 6.5 μm. The conidia were aseptate, cylindrical, with round apex and base, and 13 × 5 μm. The isolated fungus was identified as Colletotrichum sp. The isolate was deposited in Culture Collection of Microorganisms (CMAA), Jaguariúna, Brazil (CMAA 1735). The isolate was further identified by sequencing the actin (ACT), chitin synthase (CHS1), beta-tubulin (TUB) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes (Damm et al. 2019). The sequences were deposited in GenBank (accession Nos. MK495949, MK495952, MK495951 and MK495950) and revealed over 98% identity with Colletotrichum plurivorum (MG747766, MG600847, MG600984 and MK862223). Reference sequences of C. orchidearum and C. magnum species complexes were used for the alignment and the phylogenetic analyses conducted using Bayesian method clustered the isolate in the clade corresponding to Colletotrichum plurivorum. The pathogenicity of the isolate was confirmed by spraying a suspension of conidia (1 × 106 mL−1) on okra cv. Santa Cruz 47 with 4–6 leaves and the control plants were sprayed with distilled water. The plants were maintained in a dew chamber for 24 h under greenhouse at 27 °C with a 12-h-light/dark photoperiod. After 5 days, dark brown necrotic lesions were observed on the leaves of okra plants whereas control plants were all symptomless. The fungus was re-isolated, and showed the same morphological characteristics as the inoculated strain, fulfilling Koch’s postulates. C. plurivorum has been reported to infect papaya (Sun et al. 2019) and cassava (Liu et al. 2019). To our knowledge, this is the first report of Colletotrichum plurivorum causing anthracnose on okra in Brazil. |
| Observaçao: | ISSN: 1125-4653. DOI: https://doi.org/10.1007/s42161-020-00615-1 Publicação associada à Atividade SEG 04.12.04.001.00.02.016, do Projeto: Transferência e validação de tecnologias para o desenvolvimento da olericultura na região metropolitana de Belém e municípios de Altamira e Tomé-Açu-PA - (TThorta), liderado pela Embrapa Amazônia Oriental (CPATU), vigência 09/2012 a 10/2016. (ID_PAT: 24598) |
| Palavras-chave: | Ascomycetes; Fungal pathogen; Vegetable; Abelmoschus esculentus. |
| Valor JCR (2019): | 1.152 |
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| Plano de Ação: | 20.19.00.128.00.02 |
| Descrição: | HALFELD-VIEIRA, B. A.; TERAO, D.; NECHET, K. de L. First report of Geotrichum candidum causing sour-rot of melon in Brazil. Plant Disease, v. 105, n. 5, p. 1537, 2020. |
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| Conteúdo: | This is the first report of G. candidum on melon in Brazil. Sour-rot is a well-known disease of melon in other countries (Ceponis 1966) and can be listed as a potential postharvest disease for melon fruit in Brazil. |
| Observaçao: | ISSN: 0191-2917 (print), 1943-7692 (online). DOI: https://doi.org/10.1094/PDIS-11-19-2484-PDN ID Ainfo CNPMA 16904. Acesso em maio, 2022. (ID_PAT: 24545) |
| Palavras-chave: | Sour-rot. |
| Valor JCR (2019): | 3.809 |
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| Plano de Ação: | 21.17.02.001.05.06 |
| Descrição: | SOARES, M. P.; PINHEIRO, V. R.; CARDOSO, I. L.; SANTOS, A. da S.; SARTORATTO, A.; ISHIKAWA, M. M.; JONSSON, C. M.; DUARTE, M. C. T.; RANTIN, F. T.; SAMPAIO, F. G. In vitro antibacterial activity of ethanol extract of Artemisia annua and its bioactive fractions against fish pathogens. Aquaculture Research, v. 52, n. 4, p. 1797-1801, 2020. |
| Parceria/partes: | UNICAMP - Universidade Estadual de Campinas, UniFAJ - Centro Universitário de Jaguariúna, UFSCar - Universidade Federal de São Carlos - campus São Carlos, |
| Conteúdo: | Abstract: Bacterial pathogens cause significant economic losses in fish farms worldwide. The frequent use of antibiotics is a growing concern due to the adverse effects on human health and the environment. We evaluate the antibacterial activity of the Artemisia annua and its bioactive components against the Aeromonas hydrophila, Streptococcus agalactiae and Flavobacterium columnare, the main bacteria that affect fish culture. Bioactive components of the A. annua were obtained using an alcoholic extraction process, yielding 10.6% of dry material. The alcoholic extract of A. annua (ae-Aa) bioactive compounds was fractionated, obtaining the fractions F1 + 2, F3, F4, F5 and F6. Artemisinin up to 10.0 mg.ml-1 was ineffective in controlling studied bacteria. Microorganisms tested were all sensitive to ae-Aa (MIC and MBC 0.40 to 2.50 mg.ml-1). The ae-Aa components F3, F4, F5 and F6 were also effective (MIC and MBC 0.015 to 2.00 mg.ml-1). F3 showed the most effective antibacterial activity (MIC 0.015 to 0.5 mg.ml-1 and MBC 0.015 to 2.0 mg.ml-1). The ae-Aa and its bioactive components presented efficacy to inhibit growth and eliminate bacterial pathogens and can be an alternative to synthetic antibiotics for farmed fish. |
| Observaçao: | ISSN: 1355-557X. DOI: https://doi.org/10.1111/are.15026 (ID_PAT: 25409) |
| Palavras-chave: | Alcoholic extract; Artemisinin; Fish pathogenic bacteria; Minimum bactericidal concentration; Minimum inhibitory concentration. |
| Valor JCR (2019): | 1.748 |