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| Plano de Ação: | ..... |
| Descrição: | BRAGA, L. P. P.; TANENTZAP, A. J.; LEE, B.; TSAI, S. M.; RAAIJMAKERS, J. M.; MENDES, R.; MENDES, L. W. Diversity of viruses and viroids in the rhizosphere of common bean cultivars differing in resistance to the fungal root pathogen Fusarium oxysporum. Applied Soil Ecology, v. 190, 105018, 2023. |
| Parceria/partes: | National Institutes of Health, Netherlands Institute of Ecology - NIOO-KNAW, University of Cambridge, CENA/USP - Centro de Energia Nuclear na Agricultura, |
| Conteúdo: | Abstract: The rhizosphere microbiome plays a key role in plant protection against soil-borne pathogens. Plant breeding for resistance against soil-borne pathogens can alter the rhizosphere microbiome. However, most studies have focused on bacterial and fungal communities, leaving the role of the virus and viroids unassessed. Here, we tested the influence of resistance breeding on the composition of rhizosphere viruses and viroids. By analyzing metatranscriptomes from the rhizosphere of common bean (Phaseolus vulgaris) cultivars with varying resistance to the soil-borne pathogen Fusarium oxysporum, we recovered sequences representing 78 and 23 novel populations of viruses and viroids, respectively. We compared the abundances of these infectious agents across the different cultivars and found that the Fusarium-resistant cultivar harbored >1.2 times more viroids and a more different composition of viroids and viruses than less resistant plants. Given their role in interfering with host metabolism and their potential influence on plant-fungi associations, our study suggests that changes in the rhizosphere infectome are an important consideration in breeding for resistance against soil-borne pathogens. |
| Observaçao: | DOI: https://doi.org/10.1016/j.apsoil.2023.105018 (ID_PAT: 32576) |
| Palavras-chave: | Rhizosphere microbiome; Plant-microbe interactions; Infectome; Food security. |
| Valor JCR : | 4.800 |
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| Plano de Ação: | ..... |
| Descrição: | MEDEIROS, T. C. T.; DABIJA, L. G.; PARASECOLO, L.; MELO, I. S. de; MORAES, L. A. B.; IFA, D. R. Differentiation of the metabolic profile of actinobacteria isolated from the soil of the caatinga biome by paper spray mass spectrometry. Journal of Mass Spectrometry, v. 58, n. 7, article e4956, 2023. |
| Parceria/partes: | USP - Universidade de São Paulo, York University, |
| Conteúdo: | Abstract: Paper spray mass spectrometry (PS-MS) is an ambient ionization technique that allows for rapid and direct mass spectrometry analysis for a wide range of chemical compounds due to its portability, little to no sample preparation, and cost-effective materials. As applications with this technique continue to expand, the identification and discrimination of bacteria at the strain level remain a promising avenue for researchers. Although studies in the past demonstrated the applicability of PS-MS to discriminate bacteria at the strain level, no one has reported the strain-level differentiation of actinobacteria without using solvent for PS-MS. Hence, this study demonstrates that optimization of PS-MS permits the investigation and differentiation of the metabolic profiles of actinobacteria without the need for solvents, diminishing the potential for sample contamination and consequently increasing the versatility of this technique. In doing so, strains of actinobacteria (CAAT P5–21, CAAT P5–16, CAAT 8–25, CAAT P8–92, and CAAT P11–13) were grown and transferred to produce a crude growth medium. The supernatant was used for the PS-MS analyses using a Thermo Scientific LTQ mass spectrometer. Multivariate statistical analysis, including principal component analysis (PCA) and hierarchal cluster analysis (HCA), was employed to chemically distinguish the strains of bacteria. As a result, each strain of actinobacteria could be visually differentiated based on their metabolic profile. These findings demonstrate the practicability of using a liquid medium as an alternative to many other organic solvents when analyzing bacteria, making PS-MS a crucial addition to a microbiologist’s research toolkit. |
| Observaçao: | ISSN: 1076-5174 (print), 1096-9888 (online). DOI: https://doi.org/10.1002/jms.4956 Nota: no periódico, a publicação está categorizada como "Application Note". (ID_PAT: 33003) |
| Palavras-chave: | Actinobacteria; Caatinga; Paper spray; PCA; Secondary metabolites. |
| Valor JCR : | 2.300 |
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| Plano de Ação: | ..... |
| Descrição: | SANTOS, T. T. M. dos; SANTANA, M. V.; MASCARIN, G. M.; ARTHURS, S. P.; FERNANDES, P. M. Collecting young nests of Atta spp. is more efficient to establish laboratory colonies than collecting founder queens after nuptial flights. International Journal of Tropical Insect Science, v. 43, p. 1837-1841, 2023. |
| Parceria/partes: | BioBee Biological Systems Ltd., UFG - Universidade Federal de Goiás, |
| Conteúdo: | Abstract: The locations of leaf-cutting ant (Atta spp.) nests present logistical challenges for researchers, due the environmental conditions, accessibility, difficult manipulation and other complicating factors for conducting manipulative experiments. A method to circumvent this obstacle lies in establishing ant colonies in the laboratory microcosm. Our objective was to compare two methods for establishing colonies of Atta spp. in the laboratory. In one approach Atta colonies were established by collecting founder queens after their mating flights, while in second approach, founder nests (founder colonies) were obtained by collecting queens, workers, and fungal gardens. In the first method, queens found on the ground after their nuptial flights (120 in 2017; 98 in 2019) were collected and established in laboratory rearing containers. In the second method, three to five months after mating flight, we hand-collected young nests of leaf-cutting ants (94 in 2018; 71 in 2020). All materials (queen, fungal garden, and workers) were transferred into rearing units (plastic pots). Field-collected founder queens and young nests were observed for queen mortality, fungal healthiness, and overall development. Results showed that most founder queens collected from mating flights failed to establish healthy colonies. Founder queens’ mortality reached up to 98.3% in 2017, and 100% in 2020. More successful establishment of laboratory colonies was attained with field-collected young nests. In this method, we observed 83.3% and 53.5% establishment success in 2017 and 2020, respectively. Field-collection of young nests is a relatively simple and efficient method for successful establishing and rearing of Atta spp. in laboratory conditions. |
| Observaçao: | ISSN: 1742-7584 (print), 1742-7592 (online). DOI: https://doi.org/10.1007/s42690-023-01060-0 Categoria da publicação no periódico: "Short Communications". (ID_PAT: 33076) |
| Palavras-chave: | Leaf-cutting ants; Lab colony; Social insects rearing; Ant nests. |
| Valor JCR : | 1.200 |
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| Plano de Ação: | 10.23.00.039.00.02 |
| Descrição: | NOVAES, R. M. L.; AMARAL, D. R. do; MORANDI, M. A. B.; FOLEGATTI, M. I. da S.; BRANDÃO, M. Comment on “The importance of GHG emissions from land use change for biofuels in Brazil: An assessment for current and 2030 scenarios”. Resources, Conservation and Recycling, v. 201, Article 107207, 2024. |
| Parceria/partes: | KTH Royal Institute of Techonology, |
| Observaçao: | ISSN: 0921-3449, 1879-0658 (online). DOI: https://doi.org/10.1016/j.resconrec.2023.107207 ✰ In reply to Maia, R. G. T., & Bozelli, H. (2022). (ID_PAT: 33107) |
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| Valor JCR : | 13.200 |
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| Plano de Ação: | 16.00.2.50.01.16 |
| Descrição: | LIMA, M. A. de; VIEIRA, R. F.; LUIZ, A. J. B.; GALVÃO, J. A. H. CH4 and N2O fluxes during paddy rice crop development, post-harvest, and fallow. Agronomía Colombiana, v. 41, n. 1, e107053, 2023. |
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| Conteúdo: | Abstract: Paddy fields are major sources of greenhouse gases, mainly met ha ne (CH4) and nitrous oxide (N2O). Defining the sampling times for determining the average diurnal emission rates is an important step in optimizing field measurement, avoiding the inf luence of possible peaks. With this purpose, diurnal gas mea-surements (CH4 and N2O) were taken using the static chamber method during five 24 h-periods (campaigns), every 2 h, at three rice crop development stages (R2, C1 campaign; R5, C2 campaign, and R8, C3 campaign), and in post-harvest (PH, C4 campaign) and in fallow (FP, C5 campaign) periods. The CH4f luxes remained close to the average f lux both at C1 (9.4 ± 1.0 mg CH4 m-2 h-1) and C2 (10.2 ± 1.4 mg CH4 m-2 h-1), allowing the gas sampling at any time of the day, except at 5:00 p.m. when a peak was observed at C1. As the CH4 f luxes for C3, C4, and C5 were close to zero, no average value was identified. The average N2O f luxes were low at C1 (1.0 ± 5.7 μg N2O m-2 h-1) and at C4 (6.7 ± 2.6 μg N2O m-2 h-1), increasing at C2 (26.9 ± 9.3 μg N2O m-2 h-1) and C3 (21.2 ± 7.2 μg N2O m-2 h-1) and reaching higher values during the C5 campaign (73.7 ± 33.3 μg N2O m-2 h-1). In general, considering the average f lux values recorded in this study, the most appropriate times for sampling N2O during the C1, C2, C3, and C4 campaigns would be from 9 p.m. to 1 a.m. and also around 11:00 a.m. Average N2O f lows in fallow would be more likely around 11:00 p.m. and 11 a.m. |
| Observaçao: | ISSN: 0120-9965 (print), 2357-3732 (on-line). DOI: https://doi.org/10.15446/agron.colomb.v41n1.107053 Publicação associada ao Projeto em Rede: Dinâmica de Gases de efeito estufa e balanço de carbono em sistemas de produção de grãos no Brasil (código SEG/Ideare 01.11.01.002.00.00), liderado pela Embrapa Arroz e Feijão (CNPAF), vigência de 01/09/2012 a 31/08/2017. (ID_PAT: 32712) |
| Palavras-chave: | Greenhouse gases; Diurnal f lux variation; Continuous water management. |
| Valor JCR : | Não se aplica |